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86F/30C for others)

F.van der KuyP

The blotted membranes were blocked with 5% non-fat dry milk (w/v) in Tris-buffered saline with 0.1% Tween-20 (TBS-T) for 1 h at room temperature and then incubated with the following primary antibodies overnight at 4C: anti-Smad2 (1:1,000 dilution, Cell Signaling Technology), anti-phospho-Smad2 (1:1,000 dilution, Cell Signaling Technology), anti-Smad3 (1:1,000 dilution, Cell Signaling Technology), anti-phospho-Smad3 (1:1,000 dilution, Cell Signaling Technology), anti-TGF-1 (1:1000 dilution, Abcam), anti-IGF-1 (1:500 dilution, Abcam), TIMP-1 (1:500 dilution, Santa Cruz), MMP-9 (1:1000 dilution, R&D), fibronectin (1:500 dilution, Santa Cruz), E-cadherin (1:2000 dilution, CST), vimentin (1:2000 dilution, CST), and -SMA (1:2000 dilution, CST)

Although your body produces glutathione naturally in the liver, levels tend to decline with age

They give women tangible proof that something is shifting at the cellular level